Rapid and simple As(III) quantification using a turbidimetric test for themonitoring of microbial arsenic bio-transformation

Caroline Michel (1) , Catherine Joulian (1) , Hafida Tris (1) , Flavie Charnois (1) , Fabienne Battaglia-Brunet (1)

1 BRGM - Bureau de Recherches Géologiques et Minières (BRGM)

A turbidimetric test for rapid quantification of As(III) (detection limit of 3 mg/L, quantification range of 10–100 mg/L) in liquid growth medium was developed for assessing and monitoring microbial As(III)-oxidizing and As(V)-reducing activities. This test is based on As(III) chelation with pyrrolidine dithiocarbamate followed by spectrometric measurement of absorbance, and was validated by comparison with AAS quantification of As after As(III)/As(V) separation.

Domaines: Biotechnologies; Biodiversité; Sciences de l'environnement; Biodiversité et Ecologie; Ecosystèmes

Caroline Michel, Catherine Joulian, Hafida Tris, Flavie Charnois, Fabienne Battaglia-Brunet. Rapid and simple As(III) quantification using a turbidimetric test for themonitoring of microbial arsenic bio-transformation. Journal of Microbiological Methods, 2020, 177, pp.106026. ⟨10.1016/j.mimet.2020.106026⟩. ⟨hal-03517511⟩