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How solid phase extraction conditions affect suspect and non-target screening for the evaluation of surface water contamination

Abstract : Identifying organic contaminants present in aquatic environments is of major concern for water quality monitoring. New analytical and data treatment tools have been developed to meet this challenge. The sample preparation before analyses is the first crucial step to be optimized before using suspect and nontarget- screening approaches for qualitative evaluations of water pollution by contaminants from various chemical and usage families. As part of the action of Aquaref (, French reference laboratory for environmental monitoring in the Water Framework Directive), different solid phase extraction conditions (SPE, n=3 per condition) were tested towards ultrahigh pressure liquid chromatography hyphenated to high resolution mass spectrometry (UHPLC-HRMS) analysis. The SPE protocols varied according to the extraction phase (Oasis HLB or multilayer cartridge), the elution solvent (methanol or methanol and dichloromethane) and sample pH (pH 3 or 7). To ensure data quality, blank solvents, extracted blanks and quality control (QC) were also injected. The QC sample corresponds to a pool of 5 μL of each sample extract and is injected repeatedly throughout the injection sequence to monitor the analytical repeatability. By suspect screening strategy, most of the investigated compounds were found in all SPE extracts. The results revealed the presence of contaminants such as pesticides, pharmaceuticals, sweeteners, or anticorrosive agents. For pesticides, approved (e.g. diuron) and not approved (e.g. terbutryn) compounds were both detected. Concerning the pharmaceuticals, drugs acting on the nervous system were most common (e.g. oxazepam), as well as betablockers (e.g. metoprolol). The without any a priori non-target approach highlighted that the SPE conditions affect the quality and the interpretation of the data. For each sample preparation method tested the number of signals (features) detected varies, as does the number of signals associated with background noise.
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Contributeur : Anne Togola Connectez-vous pour contacter le contributeur
Soumis le : mardi 13 mars 2018 - 17:22:29
Dernière modification le : mercredi 3 août 2022 - 04:04:17


  • HAL Id : hal-01731013, version 1



Bénilde Bonnefille, C. Guillemain, Anne Togola, Coralie Soulier, Francois Lestremau, et al.. How solid phase extraction conditions affect suspect and non-target screening for the evaluation of surface water contamination. International conference on Emerging Contaminants 2018 (EmCon), Jun 2018, Oslo, Norway. 2018. ⟨hal-01731013⟩



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