Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Representative sampling of natural biofilms: influence of substratum type on the bacterial and fungal communities structure

Abstract : In situ biofilm sampling is a key step for the study of natural biofilms and using methodologies that reflect natural diversity is necessary to guarantee representative sampling. Here, we focalise on the impact of the type of substrata on which biofilms grow on bacterial and fungal communities’ structure. The indirect molecular approach, Denaturing Gel Gradient Electrophoresis (DGGE) of a gene fragment coding for either 16S rRNA or 28S rRNA, for bacteria or fungi respectively, was used to evaluate the variability of microbial community structures among different biofilm substrata: natural (pebbles, live plants, wood and sediment), or artificial (glass, Plexiglas® and sterile wood), in a small river (the Loiret, France). Multivariate statistics, band richness and diversity indexes (Shannon and Simpson) were used to highlight variations in community structure between substrata. Results showed variations of bacterial and fungal diversity between different substrata according to substratum properties/origin (natural or artificial, organic or inorganic) but there was no optimal substratum for sampling, and artificial substrata were not significantly less applicable than natural substrata. Pooling 4 different substrata types allowed a higher bacterial and fungal biodiversity recovery. Point contact sampling may thus gain in robustness by increasing the number of substrata considered. Fungal species richness was similar to the bacterial one on most substrata which suggested they should be more frequently considered in riverine biofilm studies.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [28 références]  Voir  Masquer  Télécharger
Contributeur : Anne-Marie Pouget Connectez-vous pour contacter le contributeur
Soumis le : mardi 23 janvier 2018 - 18:08:16
Dernière modification le : lundi 25 juillet 2022 - 03:49:26
Archivage à long terme le : : jeudi 24 mai 2018 - 14:32:11


Fichiers produits par l'(les) auteur(s)




Jennifer Hellal, Caroline Michel, Vanessa Barsotti, Valérie Laperche, Françis Garrido, et al.. Representative sampling of natural biofilms: influence of substratum type on the bacterial and fungal communities structure. SpringerPlus, SpringerOpen, 2016, 5 (1), ⟨10.1186/s40064-016-2448-2⟩. ⟨hal-01691299⟩



Consultations de la notice


Téléchargements de fichiers