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Article dans une revue
Arsenite-induced changes in abundance and expression of arsenite transporter and arsenite oxidase genes of a soil microbial community
Abstract : We describe a real-time PCR assay for the quantitative detection of arsB and ACR3(1) arsenite transporter gene families, two ubiquitous and key determinants of arsenic resistance in prokaryotes. The assay was applied in batch growth experiments using a wasteland soil bacterial community as an inoculum to investigate the effect of increasing arsenite [As(III)] concentrations on genes and transcript abundances. The aioA gene encoding the large subunit of arsenite oxidase was monitored in parallel. Results showed that arsB and ACR3(1) gene abundances correlated positively with the As(III) concentration. Both genes showed similar transcription patterns and strong upregulation by arsenic. Microbial As(III) oxidation occurred in As(III) spiked cultures and was associated with expression of the aioA gene in most cases. However, aioA was also expressed in several non-amended culture replicates. Analysis of cDNA clone libraries revealed that Pseudomonas was the dominant metabolically active genus whatever the As(III) concentration. Expressed arsB and ACR3(1) gene sequences were also affiliated with those from Pseudomonas, while expressed aioA sequences were more taxonomically diverse. The study suggests that arsenite transporter genes are appropriate biomarkers of arsenic stress that may be suitable for further exploring the adaptive response of bacterial communities to arsenic in contaminated environments
https://hal-brgm.archives-ouvertes.fr/hal-01026465
Contributeur : Anne-Marie Pouget Connectez-vous pour contacter le contributeur
Soumis le : lundi 21 juillet 2014 - 16:29:24
Dernière modification le : mardi 19 octobre 2021 - 19:01:17
Contributeur : Anne-Marie Pouget Connectez-vous pour contacter le contributeur
Soumis le : lundi 21 juillet 2014 - 16:29:24
Dernière modification le : mardi 19 octobre 2021 - 19:01:17