Accéder directement au contenu Accéder directement à la navigation
Communication dans un congrès

Evaluation of microbial communities genetic and functional diversity in natural biofilms to optimise growth and sampling

Abstract : A growth in the interest for microbial biofilms has been observed since the publication of numerous results showing that the majority of micro-organisms favour this mode of development in natural conditions. Indeed, their functioning and their organisation are studied in various areas such as medical applications or industries as well as environmental applications. However, because of the diversity of potential biofilm surfaces in the environment, it is particularly difficult to study natural biofilms. Thus, in order to define an adapted growth and sampling method for natural biofilms, we first evaluated the influence of the nature of natural surfaces (stone, leave, bark, plant) on the genetic and functional diversity of biofilms microbial communities in a natural river system. Then we tested the ability of artificial surfaces (glass, Plexiglas, sterile wood) to grow biofilms with genetic and functional diversities similar to those on natural surfaces. The biofilms' genetic diversity was assessed using a molecular method; Denaturing Gel Gradient Electrophoresis (DGGE) of a gene fragment coding for either 16S rRNA or 18S rRNA, for bacterial and fungi respectively, while functional diversity was estimated with ECOLOG plates (Biolog®). Results showed the importance of varying natural biofilm surfaces in order to recover a representative microbial diversity. Biofilms that developed on the artificial surfaces had similar genetic patterns between themselves, as well as similarities with the natural water samples. However these biofilms differed from the natural biofilms collected in the same sampling spot. The combination of these two approaches, using genetic and functional microbial diversity as criteria, enabled us optimize our biofilm sampling methods and adapt our biofilm culture methods. Moreover, we were able to compare the evolution of biofilms in a natural environment on different surfaces and to evaluate maximum diversity in river biofilms. Finally, this is one of the few studies that have evaluated fungal diversity in natural biofilms although in the present case it was estimated to be higher than for bacteria.
Type de document :
Communication dans un congrès
Liste complète des métadonnées

https://hal-brgm.archives-ouvertes.fr/hal-00553627
Contributeur : Anne-Marie Pouget <>
Soumis le : vendredi 7 janvier 2011 - 16:42:12
Dernière modification le : mercredi 10 juin 2020 - 16:56:15

Identifiants

  • HAL Id : hal-00553627, version 1

Collections

Citation

Jennifer Harris-Hellal, Catherine Joulian, Vanessa Barsotti, Valérie Laperche, Françis Garrido. Evaluation of microbial communities genetic and functional diversity in natural biofilms to optimise growth and sampling. 13th International Symposium on Microbial Ecology, Aug 2010, Seattle, United States. ⟨hal-00553627⟩

Partager

Métriques

Consultations de la notice

162