Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes

Abstract : Background: Thiomonas strains are ubiquitous in arsenic-contaminated environments. Differences between Thiomonas strains in the way they have adapted and respond to arsenic have never been studied in detail. For this purpose, five Thiomonas strains, that are interesting in terms of arsenic metabolism were selected: T. arsenivorans, Thiomonas spp. WJ68 and 3As are able to oxidise As(III), while Thiomonas sp. Ynys1 and T. perometabolis are not. Moreover, T. arsenivorans and 3As present interesting physiological traits, in particular that these strains are able to use As( III) as an electron donor. Results: The metabolism of carbon and arsenic was compared in the five Thiomonas strains belonging to two distinct phylogenetic groups. Greater physiological differences were found between these strains than might have been suggested by 16S rRNA/rpoA gene phylogeny, especially regarding arsenic metabolism. Physiologically, T. perometabolis and Ynys1 were unable to oxidise As( III) and were less arsenic-resistant than the other strains. Genetically, they appeared to lack the aox arsenic-oxidising genes and carried only a single ars arsenic resistance operon. Thiomonas arsenivorans belonged to a distinct phylogenetic group and increased its autotrophic metabolism when arsenic concentration increased. Differential proteomic analysis revealed that in T. arsenivorans, the rbc/cbb genes involved in the assimilation of inorganic carbon were induced in the presence of arsenic, whereas these genes were repressed in Thiomonas sp. 3As. Conclusion: Taken together, these results show that these closely related bacteria differ substantially in their response to arsenic, amongst other factors, and suggest different relationships between carbon assimilation and arsenic metabolism.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [42 références]  Voir  Masquer  Télécharger
Contributeur : Anne-Marie Pouget Connectez-vous pour contacter le contributeur
Soumis le : vendredi 27 août 2010 - 15:46:49
Dernière modification le : mercredi 3 août 2022 - 04:01:37
Archivage à long terme le : : mardi 23 octobre 2012 - 15:16:18


Fichiers éditeurs autorisés sur une archive ouverte




Christopher G. Bryan, Marie Marchal, Fabienne Battaglia-Brunet, Valerie Kugler, Christelle Lemaitre-Guillier, et al.. Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes. BMC Microbiology, BioMed Central, 2009, 9 (127), 12 p. ⟨10.1186/1471-2180-9-127⟩. ⟨hal-00512152⟩



Consultations de la notice


Téléchargements de fichiers