Comparison of corneal endothelial mosaic according to the age: the corimmo 3D project

Klervi Rannou (1, 2, 3) , Emmanuel Crouzet (4, 5) , Caroline Ronin (4, 5) , Patricio Guerrero (1, 2, 3) , Gilles Thuret (4, 5, 6) , Philippe Gain (4, 5) , Jean-Charles Pinoli (1, 2, 3) , Yann Gavet (1, 2, 3)

1 SPIN-ENSMSE - Centre Sciences des Processus Industriels et Naturels
2 PROPICE-ENSMSE - Département PROcédés Poudres, Interfaces, Cristallisation et Ecoulements
3 LGF-ENSMSE - Laboratoire Georges Friedel
4 BIIO - Biologie, Ingénierie et Imagerie pour l'Ophtalmologie
5 UJM - Université Jean Monnet - Saint-Étienne
6 IUF - Institut universitaire de France

Aim: The human corneal endothelium is a monolayer of flat hexagonal cells. It is a nearly regular hexagonal tessellation during the first years of life, but with age, becomes less regular in shape and size. The aim is to evaluate geometrically the age of an endothelial mosaic. Material and methods: Segmented endothelial mosaics of healthy subjects of different age groups are compared by morphological criteria. The mosaics are studied according to their age group (decades), their age and their location (center or mid-periphery of the cornea). The measures used are: the cell density, the Ripley's L function and the cell area and perimeter density. Results: These measures point out the endothelial cell density decrease, the cell area, perimeter and diameter increase, the cell heterogeneity increase, and the differences between central and mid-peripheral cells increases with age. Conclusion: These measures are able to characterize healthy mosaics.

Mots clés: Ripley's function; Cell morphology; Corneal endothelium; Area density; Perimeter density

Domaines: Informatique [cs]; Modélisation et simulation; Sciences de l'ingénieur [physics]; Traitement du signal et de l'image [eess.SP]; Génie des procédés

Klervi Rannou, Emmanuel Crouzet, Caroline Ronin, Patricio Guerrero, Gilles Thuret, et al.. Comparison of corneal endothelial mosaic according to the age: the corimmo 3D project. Innovation and Research in BioMedical engineering, 2016, 37 (2), pp.124-130. ⟨10.1016/j.irbm.2016.03.004⟩. ⟨hal-01365604⟩